Folate Conjugated Fluorescent Silica Nanoparticles for Labeling Neoplastic Cells
Authors: Santra, Swadeshmukul; Liesenfeld, Bernd; Dutta, Debamitra; Chatel, David; Batich, Christopher D.; Tan, Weihong; Moudgil, Brij M.; Mericle, Robert A.
Source: Journal of Nanoscience and Nanotechnology, Volume 5, Number 6, June 2005 , pp. 899-904(6)
Publisher: American Scientific Publishers
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Abstract:
We describe a novel technique of using fluorescent silica nanoparticles (FSNPs) to detect over-expressed folate receptors, as typical for certain malignancies (metastatic adenocarcinoma, pituitary adenoma and others). Using Stöber's method with some modification, 135 nm size FSNPs were synthesized by a hydrolysis and co-condensation reaction of tetraethylorthosilicate (TEOS), fluorescein labeled (3-aminopropyl)triethoxysilane (APTS) and a water-dispersible silane reagent, (3-trihydroxysilyl)propyl methylphosphonate (THPMP) in the presence of ammonium hydroxide catalyst. Folic acid (folate) was covalently attached to the amine modified FSNPs by a carbodiimide coupling reaction. The characterization of folate-FSNPs was performed using a variety of spectroscopic (UV-VIS and fluorescence), microscopic (transmission electron microscopy, TEM) and light scattering techniques. Folate conjugated FSNPs were then targeted to human squamous cancer cells (SCC-9). Laser scanning confocal images successfully demonstrated the labeling of SCC-9 cells and the efficacy of FSNP based detection system.Keywords: SILICA NANOPARTICLES; FITC; FLUORESCENCE; FOLATE; CANCER DETECTION
Document Type: Research article
DOI: 10.1166/jnn.2005.146
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