Integration of an Immunoassay System into a Microchip for High-Throughput Assay

Authors: Kiichi Satoa; Takehiko Kitamorib

Source: Journal of Nanoscience and Nanotechnology, Volume 4, Number 6, July 2004 , pp. 575-579(5)

Publisher: American Scientific Publishers

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Abstract:

An immunosorbent assay system was integrated into a glass microchip. The scale merits of liquid microspace for molecular behavior contributed remarkably to reduced assay time, and troublesome operations required for conventional immunosorbent assays could be replaced by simple operations. Moreover, a micro-immunoassay system suitable for simultaneous assay of multiple samples was constructed on a microchip. The chip had branching multichannels and four reaction and detection regions; thus the constructed system could process four samples at a time with only one pump unit. A higher throughput assay was realized with the branching structure chip.

Keywords: BEAD-BED IMMUNOASSAY; IMMUNOSORBENT ASSAY; THERMAL LENS MICROSCOPE; MICRO-FLUIDICS; MULTICHANNEL MICROCHIP

Document Type: Research article

DOI: http://dx.doi.org/10.1166/jnn.2004.016

Publication date: 2004-07-01

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Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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