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Visualizing the Self-Assembly of Tubulin with Luminescent Nanorods

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Abstract:

We succeeded in labeling tubulin with luminescent CdSe nanorods. The labeled tubulin was still able to self-assemble into microtubules, demonstrating that the protein has remained functional. The comparison with rhodamine-labeled tubulin revealed that whereas the rhodamine bleached completely within the observed time frame, the nanorods did not show any bleaching, which makes it possible to follow the key events of self-assembly at high time resolution. Preparation and derivatization of nanorods are described. Water-soluble, protein-reactive group-containing particles were covalently coupled to tubulin. The conjugate was purified by one cycle of assembly and disassembly and used to induce the formation of fluorescent microtubules. These results suggest that the use of luminescent nanorods should allow continuous confocal monitoring of dynamic biological processes.

Keywords: ANALYTICAL METHODS; COLLOIDS; FLUORESCENCE MICROSCOPY; FLUORESCENT PROBES; LUMINESCENCE; SELF-ASSEMBLY OF TUBULIN; SEMICONDUCTOR NANOPARTICLES

Document Type: Research Article

DOI: https://doi.org/10.1166/jnn.2003.219

Publication date: 2003-10-01

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  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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