Nanoporous Aluminum Oxide as a Novel Support Material for Enzyme Biosensors

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Abstract:

To construct novel amperometric sensors for the detection of hydrogen peroxide and pyruvate, peroxidase and pyruvate oxidase were immobilized in self-supporting nanoporous alumina membranes those made by anodic oxidation. Pyruvate oxidase and other enzymes were enclosed in poly(carbamoylsulfonate) hydrogel and sucked into the nanoporous alumina structure before polymerization. The alumina membranes were investigated by scanning electron microscopy before and after the enzyme immobilization. In an amperometric flow detector cell, pyruvate and hydrogen peroxide were detected under flow injection analysis conditions in concentration ranges from 1 M to 100 M and 5 M to 500 M, respectively. The achieved operational stability showed that alumina membranes can be used to construct enzyme-modified electrodes.

Keywords: AMPEROMETRIC DETECTION; ANODIC OXIDATION; ENZYME SENSORS; NANOPOROUS ALUMINA

Document Type: Research Article

DOI: http://dx.doi.org/10.1166/jnn.2003.224

Publication date: October 1, 2003

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  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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