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Nanosensor for In Vivo Measurement of the Carcinogen Benzo[a]pyrene in a Single Cell

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Abstract:

This work describes the fabrication and the application of an antibody-based fiber-optic nanosensor for in situ measurements of the carcinogen benzo[a]pyrene (BaP) in a single cell. This antibody-based spectroscopic nanosensor is miniaturized enabling the detection of fluorescent analytes in single cells. In addition to measuring fluorescent analytes in single cells, the nanosensor has the potential to be applied for both diagnostic and proteomics purposes. In this work, the human breast carcinoma cell line, MCF-7, was used as the model system to perform BaP measurements in single cells. A standard concentration curve for BaP was established and used to perform quantitative analyses of BaP in individual cells. From these analyses, it was estimated that the concentration of BaP in the individual cells investigated was ~3.61 × 10-10 M. The results obtained demonstrate the application of antibody-based nanosensors for performing in situ measurements inside a single cell.

Keywords: ANTIBODY IMMOBILIZATION; BENZO [A] PYRENE; FLUORESCENCE; NANOSENSOR; SINGLE CELL

Document Type: Research Article

DOI: https://doi.org/10.1166/jnn.2002.155

Affiliations: Advanced Biomedical Science and Technology Group, Life Sciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA

Publication date: 2002-12-01

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  • Journal for Nanoscience and Nanotechnology (JNN) is an international and multidisciplinary peer-reviewed journal with a wide-ranging coverage, consolidating research activities in all areas of nanoscience and nanotechnology into a single and unique reference source. JNN is the first cross-disciplinary journal to publish original full research articles, rapid communications of important new scientific and technological findings, timely state-of-the-art reviews with author's photo and short biography, and current research news encompassing the fundamental and applied research in all disciplines of science, engineering and medicine.
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