Skip to main content

Multifunctional Poly(amidoamine) Dendrimer-Taxol Conjugates: Synthesis, Characterization and Stability

Buy Article:

$105.00 plus tax (Refund Policy)

A series of multifunctional dendrimer-taxol conjugates have been synthesized for targeted drug delivery, based on the selected amine-terminated poly(amidoamine) dendrimer of generation 5 (PAMAM G5) as a platform. An imaging agent (fluorescein isothiocyanate), a targeting agent (folic acid), and taxol were sequentially covalently conjugated to the dendrimer. Two linkers, succinic acid and glutaric acid, were employed to attach taxol to dendrimer. The synthesized multifunctional dendrimer conjugates were extensively characterized using a variety of techniques including nuclear magnetic resonance (NMR), UV-Vis spectrometry, reverse-phase high performance liquid chromatography (RP-HPLC), and matrix-assisted laser desorption ionization-time of flight (MALDI-TOF mass spectrometry). Stability assays indicated that the two dendrimer-taxol conjugates exhibited different rates of taxol release. A kinetic study of the hydrolysis of the succinic acid linker/dendrimer conjugate using quantitative thin layer chromatography (TLC) showed that the conjugate released free taxol over time through hydrolysis in PBS buffer with a half-life of 10 hours, while no significant taxol release was observed for the glutaric acid linker/dendrimer conjugate after 7 days in the same conditions. The synthesized PAMAM dendrimer-based multifunctional dendrimer-taxol conjugates are ready for experiments in targeted cancer therapy.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics


Document Type: Research Article

Publication date: 01 September 2007

More about this publication?
  • Journal of Computational and Theoretical Nanoscience is an international peer-reviewed journal with a wide-ranging coverage, consolidates research activities in all aspects of computational and theoretical nanoscience into a single reference source. This journal offers scientists and engineers peer-reviewed research papers in all aspects of computational and theoretical nanoscience and nanotechnology in chemistry, physics, materials science, engineering and biology to publish original full papers and timely state-of-the-art reviews and short communications encompassing the fundamental and applied research.
  • Editorial Board
  • Information for Authors
  • Submit a Paper
  • Subscribe to this Title
  • Terms & Conditions
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more