Skip to main content

Adipose-Derived-Stem-Cell-Seeded Fibrin Matrices for Periodontal Ligament Engineering: The Need for Dynamic Strain

Buy Article:

$105.00 plus tax (Refund Policy)

Introduction: The periodontal ligament (PDL) connects the tooth to the alveolar bone. For PDL regeneration after tissue damage, we propose human adipose-derived stem cells (hASCs) embedded in fibrin. We showed previously that hASCs in fibrin extensively produce collagen, but in a non-functional, random orientation. Also, hASCs produce undesirable factors associated with matrix calcification. We hypothesize that with dynamic strain the matrix will align, and the calcification potential of hASCs reduces. Materials and Methods: Starting at day 0 (immediate) or day 4 (delayed), hASCs-seeded fibrin matrices were statically or dynamically strained for 3 days. Fiber and cell alignment, ECM production, and calcification-associated gene-expression were assessed. Results: Cell and fibrin fiber orientation was random in non-strained fibrin matrices. Regardless of straining type and onset, cells and fibers aligned in the strain direction. Immediate static strain decreased ALP, RUNX-2, type III collagen and elastin expression. Immediate dynamic strain decreased RUNX-2 and osteonectin expression. Delayed strain hardly affected gene expression. Discussion: Provided that dynamic loading commences immediately, expression of calcificationassociated genes by hASCs reduces, while matrix and cells align without compromising their regenerative capacity. These results combined confirm the potential for hASC-seeded fibrin matrices for PDL regeneration.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Keywords: BIOMECHANICS; CELL DIFFERENTIATION; PERIODONTAL LIGAMENT (PDL); REGENERATIVE MEDICINE; STEM CELL(S); TISSUE ENGINEERING

Document Type: Research Article

Publication date: 01 December 2017

More about this publication?
  • Journal of Biomaterials and Tissue Engineering (JBT) is an international peer-reviewed journal that covers all aspects of biomaterials, tissue engineering and regenerative medicine. The journal focuses on the broad spectrum of research topics including all types of biomaterials, their properties, bioimplants and medical devices, biofilms, bioimaging, BioMEMS/NEMS, biosensors, fibers, tissue scaffolds, tissue engineering and modeling, artificial organs, tissue interfaces, interactions between biomaterials, blood, cells, tissues, and organs, regenerative medicine and clinical performance.
  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more