Insulin-Loaded Poly(ε-Caprolactone) Nanoparticles: Efficient, Sustained and Safe Insulin Delivery System
The aim of this work was to develop an efficient, biodegradable, biocompatible and safe controlled release system using insulin-loaded poly( -caprolactone) (PCL) nanoparticles. The insulin-loaded PCL nanoparticles were prepared by double emulsion method (water-in-oil-in-water) using
Pluronic F68 as emulsifier. Using the double emulsion method a high insulin encapsulation efficiency (90.6 ±1.6%) with a zeta potential of –29±2.7 mV and average particle size of 796±10.5 nm was obtained. Insulin-loaded PCL nanoparticles showed no toxicity to MIN6
cells. Insulin nanoparticles administered subcutaneously and intraperitoneally in rats reduced glycaemia of basal levels after 15 minutes, and presented a sustainable hypoglycemic effect on insulin-dependent type 1 diabetic rats, showing to be more efficient than unencapsulated insulin. Furthermore,
these nanoparticles were not hepatotoxic, as evaluated by the effect over liver cell-death and oxidative stress scavenger system in rats. These results suggest that insulin-loaded PCL nanoparticles prepared by water-in-oil-in-water emulsion method are biocompatible, efficient and safe insulin-delivering
system with controlled insulin release, which indicates that it may be a powerful tool for insulin-dependent patients care.
Keywords: CYTOTOXICITY; DIABETES; HEPATOTOXICITY; INSULIN; POLYMERIC NANOPARTICLES; SUSTAINED RELEASE
Document Type: Research Article
Publication date: 01 June 2013
- Journal of Biomedical Nanotechnology (JBN) is a peer-reviewed multidisciplinary journal providing broad coverage in all research areas focused on the applications of nanotechnology in medicine, drug delivery systems, infectious disease, biomedical sciences, biotechnology, and all other related fields of life sciences.
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