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Electrochemical Detection of E. coli O157:H7 Using Porous Pseudo-Carbon Paste Electrode Modified with Carboxylic Multi-Walled Carbon Nanotubes, Glutaraldehyde and 3-Aminopropyltriethoxysilane

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Abstract:

Fabrication of three different electrodes based on functional porous pseudo-carbon paste electrodes (PPCPEs) was described. PPCPEs were modified with carboxylic multi-walled carbon nanotubes (PPCPE-COOH), glutaraldehyde (PPCPE-CHO) and 3-aminopropyltriethoxysilane (PPCPE-NH2. The modified electrodes were applied in detection of E. coli O157:H7, it was showed that the electrochemical signal of PPCPE-CHO was the strongest among those three kinds of electrodes. A linear relationship between the anodic stripping peak current and the concentration of E. coli O157:H7 from 1.0 × 103 to 1.0 × 107 cells/mL and a limit of detection as low as 8.0 × 102 cells/mL were obtained when PPCPE-CHO was used.

Keywords: 3-AMINOPROPYLTRIETHOXYSILANE; CARBOXYLIC MULTI-WALLED CARBON NANOTUBES; ELECTROCHEMICAL BIOSENSOR; POROUS PSEUDO-CARBON PASTE ELECTRODE

Document Type: Research Article

DOI: http://dx.doi.org/10.1166/jbn.2012.1456

Publication date: December 1, 2012

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  • Journal of Biomedical Nanotechnology (JBN) is a peer-reviewed multidisciplinary journal providing broad coverage in all research areas focused on the applications of nanotechnology in medicine, drug delivery systems, infectious disease, biomedical sciences, biotechnology, and all other related fields of life sciences.
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