Preparation and Characterization of Monodisperse Core–Shell Fe3O4 @SiO2 Microspheres and Its Application for Magnetic Separation of Nucleic Acids from E. coli BL21
Abstract:In this article, we present an easy route to prepare monodisperse core–shell Fe3O4 @SiO2 microspheres with uniform size and shape. Their structures and properties were studied by transmission electron microscopy (TEM), scanning electron microscopy (SEM), Fourier transform infrared spectrometry (FT-IR), and vibrating sample magnetometer (VSM), respectively. The results showed that spherical Fe3O4 microspheres with well dispersion have a rough surface and an average diameter (about 500 nm). After the modification with silica, the particles have a well-defined core–shell structure and a much smoother surface and larger particle diameter (about 600 nm). Furthermore, VSM measurements indicated that the as-prepared Fe3O4 and Fe3O4 @SiO2 microspheres were superparamagnetic at room temperature and the saturation magnetization (M s ) were 58.110 emu/g and 33.479 emu/g, respectively. And then, the prepared monodisperse core–shell Fe3O4 @SiO2 microspheres were subsequently applied to separate nucleic acids from the bacteria (E. coli BL21) and verified the great application prospects for bioseparation technology of the biomoleculars.
Document Type: Research Article
Publication date: 2012-12-01
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- Journal of Biomedical Nanotechnology (JBN) is a peer-reviewed multidisciplinary journal providing broad coverage in all research areas focused on the applications of nanotechnology in medicine, drug delivery systems, infectious disease, biomedical sciences, biotechnology, and all other related fields of life sciences.
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