In Vivo Evaluation of Curcumin Loaded Nanosuspensions by Oral Administration
Abstract:The current study was aimed at preparing the curcumin nanosuspension (CUR-NS) to improve the solubility and oral absorption of CUR. Prepared from a tandem of ultra-turrax homogenization and high pressure homogenization, the CUR-NS showed spherical shape under transmission electron microscopy with an average diameter of 210.2 nm. The absorption of CUR-NS in the gastrointestinal (GI) tract was studied in rats using an in situ single pass perfusion method. It was found that the absorption percentage in stomach was 9.20% within 2 h. The absorption process in intestine was first-process with passive diffusion mechanism, and the main absorptive segments were proven to be in the duodenum and jejunum. A pharmacokinetic study was conducted in mice after oral administration of CUR at 250 mg/kg in the form of either CUR-NS or CUR suspension. The plasma concentration-time curves were both fitted to a one-compartment model and the relative bioavailability of CUR-NS to CUR suspension was 680.03%. The effects of CUR-NS on the structural properties of the intestinal mucosal membrane of rats were investigated by fluorescence polarization and circular dichroism in vitro. After treatment with CUR-NS, not only the fluidity of intestinal mucosal membrane increased but also the conformation of the membrane protein loosed, which increased the gastrointestinal absorption of CUR-NS. These studies provided the evidence that NS was valuable as an oral delivery carrier to enhance the absorption of CUR.
Document Type: Research Article
Publication date: August 1, 2012
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- Journal of Biomedical Nanotechnology (JBN) is a peer-reviewed multidisciplinary journal providing broad coverage in all research areas focused on the applications of nanotechnology in medicine, drug delivery systems, infectious disease, biomedical sciences, biotechnology, and all other related fields of life sciences.
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