@article {Anitha:2012:1550-7033:29,
title = "5-Flourouracil Loaded N,O-Carboxymethyl Chitosan Nanoparticles as an Anticancer Nanomedicine for Breast Cancer",
journal = "Journal of Biomedical Nanotechnology",
parent_itemid = "infobike://asp/jbn",
publishercode ="asp",
year = "2012",
volume = "8",
number = "1",
publication date ="2012-02-01T00:00:00",
pages = "29-42",
itemtype = "ARTICLE",
issn = "1550-7033",
url = "https://www.ingentaconnect.com/content/asp/jbn/2012/00000008/00000001/art00004",
doi = "doi:10.1166/jbn.2012.1365",
keyword = "5-FLUOROURACIL, BREAST CANCER, N,O-CARBOXYMETHYL CHITOSAN, NANOPARTICLES, CANCER DRUG DELIVERY, BIOMATERIALS",
author = "Anitha, A. and Chennazhi, K. P. and Nair, S. V. and Jayakumar, R.",
abstract = "Chitosan and its carboxymethyl derivatives are smart biopolymers that are non-toxic, biocompatible, biodegradable and hence found applications in biomedical field. In the current work, we have developed 5-fluorouracil (5-FU) loaded N,O-carboxymethyl chitosan (N,O-CMC)
nanoparticles (mean diameter: 80\textpm20 nm, zeta potential: +52.47\textpm2 mV) for cancer drug delivery. Drug entrapment efficiency (65%) and in vitro drug release studies were carried out spectrophotometricaly. Cellular internalization of the drug loaded nanoparticles was confirmed
by fluorescent microscopy and flow cytometric analysis. Results of anticancer activity via MTT, apoptosis and caspase 3 assays showed the toxicity of the drug loaded nanoparticles towards breast cancer cells. As a whole these results indicates the potential of 5-FU loaded N,O-CMC nanoparticles
in breast cancer chemotherapy in which the side effects of conventional chemo treatment could be reduced. Furthermore, the results of in vitro hemolytic assay and coagulation assay substantiate the blood compatibility of the system as well.",
}