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Capture of Enveloped Viruses Using Polymer Tentacles Containing Magnetic Latex Particles

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Abstract:

A new rapid and efficient method for enveloped viruses' capture, purification and concentration has been developed. The approach is based on the use of functionalized magnetic latex particles as a carrier for capturing of the biological samples. After the capturing step, the magnetic particles are rapidly separated from the supernatant via simple permanent magnet. The nucleic acids are then directly extracted from the captured viruses on the magnetic particles using a commercial kit. The concentration methodology of viruses was first optimized for low-concentrated samples without loss of viral activity and infectivity (detected by HIV-1 p24 antigen production assay). Sensitivity of the capture assay was determined using PCR or RT-PCR method and the detection limit was found to be 10 and 16 copies for HGV and HBV respectively. The capture assay has been further used in the subtraction hybridization technique for identification of differentially expressed sequences. Finally, the lack of the tester and driver samples from the same patient by “viral positivation” of a negative serum has been circumvented using viral capture and transfer.

Keywords: BIOMEDICAL; BIOTECHNOLOGY; CAPTURE; DETECTION; MAGNETIC PARTICLES; PURIFICATION; SUBTRACTION HYBRIDIZATION; VIRUS

Document Type: Research Article

DOI: http://dx.doi.org/10.1166/jbn.2010.1093

Publication date: February 1, 2010

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  • Journal of Biomedical Nanotechnology (JBN) is a peer-reviewed multidisciplinary journal providing broad coverage in all research areas focused on the applications of nanotechnology in medicine, drug delivery systems, infectious disease, biomedical sciences, biotechnology, and all other related fields of life sciences.
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