Skip to main content

Polymeric Nanosensors for Measuring the Full Dynamic pH Range of Endosomes and Lysosomes in Mammalian Cells

Buy Article:

$113.00 plus tax (Refund Policy)

Abstract:

Polymer nanoparticle sensors have been constructed for studying pH in the endocytic pathway in mammalian cells. The pH sensors for fluorescence ratiometric measurements were prepared using inverse microemulsion polymerization with rhodamine as reference fluorophor and fluorescein and oregon green as pH sensitive dyes, which gave a dynamic pH measurement range from 4.1–7.5. Thus, the sensors cover the pH range of almost all intracellular compartments in mammalian cells. Both neutral and cationic polyacrylamide particles were synthesized where (3-acrylamidopropyl) trimethylammonium chloride was used to introduce a net positive charge in the cationic particles. It was found that the positively charged particle sensors were internalized spontaneously by HepG2 cancer cells. These new pH nanosensors are potential tools in time resolved quantification of pH in the endocytic pathway of living cells.

Keywords: ENDOSOME; LYSOSOME; NANOPARTICLE; NANOSENSOR; PH SENSOR; POLYACRYLAMIDE

Document Type: Research Article

DOI: http://dx.doi.org/10.1166/jbn.2009.1084

Publication date: December 1, 2009

More about this publication?
  • Journal of Biomedical Nanotechnology (JBN) is a peer-reviewed multidisciplinary journal providing broad coverage in all research areas focused on the applications of nanotechnology in medicine, drug delivery systems, infectious disease, biomedical sciences, biotechnology, and all other related fields of life sciences.
  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • Terms & Conditions
  • Ingenta Connect is not responsible for the content or availability of external websites

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Partial Open Access Content
Partial Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more