Skip to main content

Intra/Extracellular Biosynthesis of Silver Nanoparticles by an Autochthonous Strain of Proteus mirabilis Isolated fromPhotographic Waste

Buy Article:

$113.00 plus tax (Refund Policy)

Abstract:

There is an enormous interest in developing green synthesis procedures for production of nanoparticles by using biomimetic approaches. In our research focus has been given to the development of an efficient and eco-friendly viable process for the synthesis of nanoscale silver particles using Proteus mirabilis PTCC 1710, a bacterial strain that was isolated during a screening program from photographic waste. A significant result of this study is our observation that silver nanoparticles could be induced to synthesis intra and extracellulary. The silver nanoparticles were characterized by means of UV–Vis spectroscopy, transmission electron microscopy (TEM), energy–dispersive spectroscopy (EDS) and atomic absorption spectrometer (AAS). The nanoparticles exhibited maximum absorbance at 400–410 nm in UV–Vis spectroscopy. TEM images showed formation of stable silver nanoparticles of spherical shape with most of the particles in the size range of 10–20 nm. It was found that incubation of bacterial cells in Muller-Hinton broth medium resulted in higher extracellular synthesis of silver nanoparticles, whereas intracellular synthesis of silver nanoparticles effectively increased in tryptic soy broth. The method of extraction of intracellular silver nanoparticles was inexpensive, simple and effective in large scale with no need to complex instruments. The bacteria work as a bionanofactory which continued to grow after synthesis of silver nanoparticles. The silver reduction by this strain is occurred through energy-dependent processes that lead to the high output of this reaction. Hence this newapproach of using a non-pathogenic bacterial strain for the successful synthesis of nanosized silvers could be easily scaled up which establishes its commercial viability.

Keywords: BIOSYNTHESIS; NANOPARTICLES; PROTEUS MIRABILIS; SILVER; TEM

Document Type: Research Article

DOI: http://dx.doi.org/10.1166/jbn.2009.1029

Publication date: June 1, 2009

More about this publication?
  • Journal of Biomedical Nanotechnology (JBN) is a peer-reviewed multidisciplinary journal providing broad coverage in all research areas focused on the applications of nanotechnology in medicine, drug delivery systems, infectious disease, biomedical sciences, biotechnology, and all other related fields of life sciences.
  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • Terms & Conditions
  • Ingenta Connect is not responsible for the content or availability of external websites
asp/jbn/2009/00000005/00000003/art00003
dcterms_title,dcterms_description,pub_keyword
6
5
20
40
5

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more