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Aminodextran Containing Magnetite Nanoparticles for Molecular Biology Applications: Preparation and Evaluation

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Within the framework of medical diagnosis, the main objective is the development of hydrophilic magnetic particles for the generic capture of the nucleic acids in order to enhance the sensitivity. The strategy used in this work is based on the synthesis of cationic and hydrophilic magnetic nanoparticles bearing aminodextran. The synthesis was performed using two different processes: (i) coprecipitation of the ferrous and ferric salts in the presence of an aqueous solution of aminodextran and (ii) via adsorption of aminodextran on iron oxide nanoparticles. The obtained particles are characterized and evaluated in non-specific nucleic acids extraction and amplification.
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Keywords: AMINODEXTRAN; AMPLIFICATION; CAPTURE OF NUCLEIC ACIDS; DIAGNOSIS; DNA; HYDROPHILIC MAGNETITE; MAGNETIC NANOPARTICLES; RNA

Document Type: Research Article

Publication date: 2009-04-01

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  • Journal of Biomedical Nanotechnology (JBN) is a peer-reviewed multidisciplinary journal providing broad coverage in all research areas focused on the applications of nanotechnology in medicine, drug delivery systems, infectious disease, biomedical sciences, biotechnology, and all other related fields of life sciences.
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