Skip to main content

Insulin-Loaded PLGA Nanoparticles for Oral Administration: An In Vitro Physico-Chemical Characterization

Buy Article:

$113.00 plus tax (Refund Policy)


The aim of this work was to study poly(d,l-lactic-co-glycolic) acid (PLGA) nanoparticles – formulated by a modified solvent diffusion technique – applied as model nanocarriers for insulin in potential oral administrations. These nanostructures consisted of a blend matrix formed by PLGA copolymer and polyoxyethylene derivatives. Two types of blend formulations, PLGA:poloxamer (Pluronic F68®) and PLGA:poloxamine (Tetronic T904®), were analyzed, and the results compared to those obtained with pure PLGA nanoparticles. The work has been divided into two parts. (a) Firstly, the stability of the unloaded nanoparticles in simulated gastric and intestinal fluids was studied. Degradation studies reflected a strong interaction between the pure PLGA nanoparticles and the digestive enzymes. However, this interaction was considerably reduced in the blend formulations, although the PLGA:poloxamine system became colloidally unstable in the simulated gastric fluid. (b) Secondly, the effect of the net charge of the encapsulated macromolecule in the final properties of the blend formulations was studied by encapsulating insulin below and above its corresponding isoelectric point. The net charge of the encapsulated protein showed a clear effect in the final size of the nanoparticles, while the encapsulation efficiency was controlled by the polyoxyethylene derivative presents in the blend formulation. The obtained results show that those carriers formed with encapsulated insulin in PLGA-Pluronic F68® particles are capable, at least in vitro, to overcome the gastrointestinal barrier. Therefore, these nanocarriers seem to be appropriate for oral administration of insulin, although performing in vivo studies becomes necessary to corroborate such statement.


Document Type: Research Article


Publication date: 2009-02-01

More about this publication?
  • Journal of Biomedical Nanotechnology (JBN) is a peer-reviewed multidisciplinary journal providing broad coverage in all research areas focused on the applications of nanotechnology in medicine, drug delivery systems, infectious disease, biomedical sciences, biotechnology, and all other related fields of life sciences.
  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • Terms & Conditions
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more