Efficacy of He-Ne Laser Irradiation on the Improvement of Biodesulfurizing Activity of Gordonia sp. WQ-01
Low-energy laser has been widely applied in breeding of microorganisms, treatment of wound healing, and cancer therapy for many years, but the molecular mechanisms of the laser irradiation are still unclear. In this study, the biological effects of the He-Ne laser irradiation on the dibenzothiophene monooxygenase (DszC) were analyzed, comparing the wild-type bacteria strain of Gordonia sp. WQ-01 and its He-Ne laser irradiated strain WQ-01A. First, the gene and protein sequences of the DszC were analyzed. The results indicated that there were ten point mutations on the dszC gene sequences, and four of them further caused alteration of four amino acid residues in DszC protein. Then, the enzymatic activities of the DszC were analyzed. It was indicated that the enzymatic activity of the mutant protein DszC was higher than wild-type protein DszC. Finally, the biodesulfurization (BDS) capacities of both the wild-type strain and the mutant strain in aqueous model oil and light gas oil systems were analyzed. The results indicated that biodesulfurization capacities of the mutant strain WQ-01A were enhanced in all the above systems. Therefore, the He-Ne laser irradiation caused the point mutation in the dszC and alteration of amino acid residues of DszC, and further enhanced the BDS capacities of the mutant strain WQ-01A.
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Document Type: Research Article
Publication date: 2008-12-01
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