Intracellular Fate of a Targeted Delivery System
Abstract:Targeted delivery of a drug to the malignant cells should result in enhanced therapeutic efficacy with low to minimal side effects. This is a widely accepted concept, but limited in application due to lack of available technologies and process of validation. Therefore, fabrication of a "smart" targeted delivery system with a tunable release profile of drug in a particular cellular compartment constitutes an essential component in targeted delivery. Nanotechnology can play an important role in this aspect. In this article, we report the investigation on the intracellular fate of such a targeted delivery system containing gold nanoparticle (AuNP) as a delivery vehicle, gemcitabine (Gem) as a cytotoxic drug and cetuximab (C225) as a targeting agent. To confirm the intracellular uptake of the nanoconjugates, we determined the intracellular gemcitabine triphosphate (GemTP) concentration of AsPC-1 cells under targeted and non-targeted condition. Furthermore, we have performed transmission electron microscopic (TEM) analysis of AsPC-1 cells (a pancreatic cancer cell line with a high degree of EGFR expression) after treatment with Au-C225-Gem and its non-targeted counterpart Au-IgG-Gem to confirm the intracellular uptake and determine the intracellular localizations of the nanoconjugates. Stability of Au-C225-Gem is studied in terms of the release of C225 and Gem under different settings such as in the cell growth media, in mouse plasma, under intracellular GSH concentration and finally in endosomal acidic environment. Results obtained from all of the experiments described above suggest that the nanoconjugate is significantly stable outside the cell both under in vitro and in vivo setting. However, the nanconjugate dissociates under intracellular environment at high GSH concentration and at acidic endosomal pH releasing functionally active Gem. These studies will have significant impact on rationally designing a nanoconjugate for a successful targeted delivery.
Document Type: Research Article
Publication date: 2008-12-01
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- Journal of Biomedical Nanotechnology (JBN) is a peer-reviewed multidisciplinary journal providing broad coverage in all research areas focused on the applications of nanotechnology in medicine, drug delivery systems, infectious disease, biomedical sciences, biotechnology, and all other related fields of life sciences.
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