Skip to main content

Microfluidic Preparation of Chlorambucil Nanoemulsion Formulations and Evaluation of Cytotoxicity and Pro-Apoptotic Activity in Tumor Cells

Buy Article:

$105.00 plus tax (Refund Policy)

The objective of this study was to formulate chlorambucil, a lipophilic DNA alkylating agent, in soybean oil-containing nanoemulsion formulations for improved delivery efficiency in solid tumors. The nanoemulsions were prepared using a high pressure homogenization method with a Microfluidizer® M-110EH processor. The optimized nanoemulsion formulations were investigated for cellular uptake, cytotoxicity, and apoptotic activity in SKOV3 human ovarian adenocarcinoma cells. The Microfludizer® processing conditions were optimized (12,500 psi for 30 sec) to obtain the average oil droplet size below 150 nm in diameter. The control, poly(ethylene glycol) (PEG)-modified, and cationic lipid (i.e., DOTAP)-modified nanoemulsion formulations had excellent chlorambucil encapsulation efficiency (>97%). The cytotoxicity and the pro-apoptotic activity of chlorambucil were significantly enhanced when administered in the nanoemulsion formulations relatively to aqueous solution. The results of this study show that stable oil-in-water nanoemulsion formulations can be prepared with a commercially-available Microfluidizer® processor. The nanoemulsion formulations were effective in intracellular delivery of chlorambucil, which enhanced the therapeutic effect in tumor cells.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Data/Media
No Metrics

Keywords: APOPTOTIC ACTIVITY; CHLORAMBUCIL; CYTOTOXICITY; MICROFLUIDIZER®; OIL-IN-WATER NANOEMULSION; OVARIAN ADENOCARCINOMA CELLS

Document Type: Research Article

Publication date: 2008-06-01

More about this publication?
  • Journal of Biomedical Nanotechnology (JBN) is a peer-reviewed multidisciplinary journal providing broad coverage in all research areas focused on the applications of nanotechnology in medicine, drug delivery systems, infectious disease, biomedical sciences, biotechnology, and all other related fields of life sciences.
  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • Terms & Conditions
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more