If you are experiencing problems downloading PDF or HTML fulltext, our helpdesk recommend clearing your browser cache and trying again. If you need help in clearing your cache, please click here . Still need help? Email help@ingentaconnect.com

Aptamer-Functionalized Au Nanoparticles for the Electrochemical Detection of Thrombin

$113.00 plus tax (Refund Policy)

Buy Article:

Abstract:

An aptamer-based sandwich assay with electrochemical detection for thrombin analysis is reported using Au nanoparticles. The primary aptamer was immobilized on the surface of a screen-printed carbon electrode (SPCE) and the secondary aptamer was immobilized on Au nanoparticles. The electrochemical reduction current response of Au nanoparticles was monitored for the quantitative detection of thrombin. The limit of detection was determined as 1 nM. The proposed work demonstrates the high specificity of aptamers together with the use of Au nanoparticles. The effect of interfering proteins such as bovine serum albumin (BSA) was investigated. Control experiments also involved the use of an aptamer that has a binding affinity to immunoglobulin E (IgE). Aptamer-based analysis using Au nanoparticles opens the possibility of applications in complex biological fluids and a step closer towards diagnostics or medical investigation.

Keywords: APTAMER; AU NANOPARTICLES; BIOSENSOR; ELECTROCHEMICAL; THROMBIN

Document Type: Research Article

DOI: http://dx.doi.org/10.1166/jbn.2008.013

Publication date: June 1, 2008

More about this publication?
  • Journal of Biomedical Nanotechnology (JBN) is a peer-reviewed multidisciplinary journal providing broad coverage in all research areas focused on the applications of nanotechnology in medicine, drug delivery systems, infectious disease, biomedical sciences, biotechnology, and all other related fields of life sciences.
  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • Terms & Conditions
  • ingentaconnect is not responsible for the content or availability of external websites
Related content

Tools

Favourites

Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more