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Analysis of Mesocavity DNA Biochip for Respiratory Syncytial Virus (RSV) Diagnosis

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Abstract:

Nanoscale biochips, based on DNA, are highly selective for biological molecules to diagnose various diseases and disorders with high sensitivity and specificity. A novel optical and mechanical approach to diagnose RSV infections has been developed by preparing a surface of porous silicon composed of mesocavities attached with RSV specific single strand DNA (ssDNA) followed by its hybridization with complementary DNA (cDNA). DNA biochip prototypes were fabricated by electro-chemical etching of silicon wafer consisting of mesocavities with a porosity of 9%. ssDNA specific to RSV genome were then attached to the mesocavities using tetra-ethyl-ortho-silicate (TEOS) as a cross linker. Hybridization of ssDNA with its cDNA probe containing fluorescence molecule was investigated. We characterized the DNA biochip and confirmed the DNA attachment and its hybridization using different techniques. Scanning electron microscopy was employed to determine the silicon pore distribution. Optical microscopy was used to confirm the detection scheme. Furthermore, photoluminescence studies confirmed the DNA hybridization by detecting blue and green light emitted throughout the visible region when excited by high energy photons. AFM spectroscopy studies of ssDNA provided monitoring of specific interactions and binding phenomena between the DNA strands and porous silicon. This lead to a calculated efficiency for exposed ssDNA of 34.5%. The fabricated DNA biochip was found to be highly stable at refrigerated condition at about 4 °C for more then 8 weeks. This work presents an enabling technology which applies low processing temperature to fabricate a DNA chip for RSV and potentially other types of biological applications.

Keywords: DNA BIOCHIP; DNA IMMOBILIZATION; DNA NANOSENSOR; PHOTOLUMINESCENCE; POROUS SILICON; RSV

Document Type: Research Article

DOI: http://dx.doi.org/10.1166/jbn.2007.020

Publication date: June 1, 2007

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  • Journal of Biomedical Nanotechnology (JBN) is a peer-reviewed multidisciplinary journal providing broad coverage in all research areas focused on the applications of nanotechnology in medicine, drug delivery systems, infectious disease, biomedical sciences, biotechnology, and all other related fields of life sciences.
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