If you are experiencing problems downloading PDF or HTML fulltext, our helpdesk recommend clearing your browser cache and trying again. If you need help in clearing your cache, please click here . Still need help? Email help@ingentaconnect.com

Intraperitoneal Administration of Calcium Phosphate Nanoparticles Encapsulating pSVgal Elicits Immune Response to Encoded Protein

$113.00 plus tax (Refund Policy)

Buy Article:

Abstract:

Calcium Phosphate (CAP) nanoparticles encapsulating a marker plasmid, pSVgal, has been administered intraperitonially to six to eight-week old female Swiss albino mice having body weight of around 20–25 g. As a consequence of pDNA expression, high concentration of -gal protein has been observed which depicts calcium phosphate nanoparticles to be an efficient non-viral vector for delivering pDNA to cells and its intracellular trafficking to the nucleus. A 10-fold increase in the antibody titers in the 6th week predominated by IgG2a over intraperitoneal injections of naked pSV gal alone. IgG2a antibody titers elevation was found to be continued in animals from 6th to 8th week duration also, and the kinetics of the anti -gal response induced by gene transfection showed about 30-fold higher antibody titers at the end of the 8th week after last immunization. Highly restricted IgG2a antibody response to the encoded antigen together with Type I cytokines secretion from in vitro -gal stimulated splenic CD4+ T cells from mice immunized with encapsulated plasmid demonstrated that immunization with CAP nanoparticles encapsulated plasmid DNA encoding protein is a powerful tool for initiating a Th1 response to that antigen. The rise in the circulating immune complex in serum of the experimental animals when compared to the control animals also supported this fact. By intraperitoneally administering pSVgal loaded CAP nanoparticles into the mice did not show any adverse local or systemic reactions in response to calcium phosphate and even after repeated doses they were very well tolerated. The total amount of pDNA administered in the animal in the present experiment for significant level of immune response was as small as 1 g only which is much lower than that administered through other carriers.
More about this publication?
  • Journal of Biomedical Nanotechnology (JBN) is a peer-reviewed multidisciplinary journal providing broad coverage in all research areas focused on the applications of nanotechnology in medicine, drug delivery systems, infectious disease, biomedical sciences, biotechnology, and all other related fields of life sciences.
  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • Terms & Conditions
  • ingentaconnect is not responsible for the content or availability of external websites
Related content

Tools

Favourites

Share Content

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more