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Loading and Release Studies of Proteins Using Poly(N-isopropylacrylamide) Based Nanogels

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Abstract:

In order to point out the driven forces in the loading and release of protein onto thermally sensitive poly(N-isopropylacrylamide) (NIPAM) nanogel particles, which largely claimed in drug delivery and analytical processes, various polyNIPAM based nanogel particles were prepared, and the loading and release of two model proteins: bovine serum albumin (BSA) and -Globulins (G), onto various cationically or anionically charged polyNIPAM nanocrogel particles were investigated. At first, the nanogels particles were well characterized regarding their colloidal properties (swelling ability, particle size, and electrokinetic properties). Then, the loading and release of proteins behaviour onto the elaborated particles were investigated as a function of temperature, pH, and salinity. It was found that the loaded amount of both proteins increases as a function of temperature irrespective of the nature of charge of polyNIPAM nanogels. The protein loaded amount onto the two lower charged polyNIPAM nanogels without functional monomers is much less than that of the two higher charged polyNIPAM nanogels with functional monomers. Moreover, the salinity and pH was found to significantly affect the loaded amount of both proteins. The loaded proteins above the volume phase transition temperature (TVPT are completely released while decreasing temperature to below TVPT, and the protein loading on polyNIPAM nanogel particles is reversible.

Keywords: LOADING; NANOGEL; POLYNIPAM; PROTEIN; RELEASE; THERMALLY-SENSITIVE

Document Type: Research Article

DOI: https://doi.org/10.1166/jbn.2006.030

Publication date: 2006-10-01

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  • Journal of Biomedical Nanotechnology (JBN) is a peer-reviewed multidisciplinary journal providing broad coverage in all research areas focused on the applications of nanotechnology in medicine, drug delivery systems, infectious disease, biomedical sciences, biotechnology, and all other related fields of life sciences.
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