Fibronectin Bonding to Nanowires and Their Internalization by Epithelial Cells
Authors: Beaux, Miles F.; Wang, Lidong; Zhang, Daqing; Gangadean, Devananda; McIlroy, David N.; Kwon, Nam Hoon; Dziewanowska, Katarzyna; Bohach, Gregory A.
Source: Journal of Biomedical Nanotechnology, Volume 2, Number 1, April 2006 , pp. 23-28(6)
Publisher: American Scientific Publishers
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Abstract:
In this study, a process was developed for introducing silica based nanowires into bovine mammary gland epithelial cells that exploits receptor/ligand interactions employed by several intracellular bacteria. Fibronectin was used as a molecular bridge to induce internalization of the nanowires into the viable epithelial cells. The bonding mode of fibronectin to silica based nanowires has been examined using X-ray photoelectron spectroscopy. Examination of the Si 2p, O 1s, and C 1s core level states before and after exposure of the nanowires to fibronectin showed that bonding of fibronectin primarily involves the Si sites on the nanowire surface. Imaging of the epithelial cells after exposure to the fibronectin coated silica nanowires with a field emission scanning electron microscope operated in the secondary electron mode and backscattered electron mode verified that the fibronectin coated nanowires are internalized by the MAC-T cells. Fibronectin enhances the rate of internalization by 50% over bare nanowires.Keywords: ENDOCYTOSIS; INTERNALIZATION; NANOWIRE; DRUG DELIVERY; FIBRONECTIN; XPS; SEM
Document Type: Research article
DOI: 10.1166/jbn.2006.001
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