Skip to main content

Beads Screening Method of Inhibitors to Amyloid--Protein Aggregation

Buy Article:

$113.00 plus tax (Refund Policy)

Abstract:

We have established a simple and fast screening method of potent inhibitors to amyloid--protein (A) aggregation using a new beads technology. It is known that fibril formation of A obeys the nucleation dependent polymerization model in which small nuclei are slowly formed and fast fibril formation of A proceeds subsequently. Congo red and thioflavin T bind in specific and regular fashions to amyloid fibrils possessing -sheet structures, which results in spectral changes and fluorescence emission, respectively. Therefore, these compounds have been used for determination of amyloid fibril formation. However, there is a long delay time before the fibril formation starts, because the formation of nuclei is very slow. This means that it takes a long time to evaluate inhibitors of fibril formation in the conventional methods using Congo red and thioflavin T. In contrast to these conventional methods, the present new method using beads is thought to detect the early stage of A aggregation, since the A-immobilized beads can easily coagulate by cross-linking with free A in solution. In this regard, the present beads method is superior to the conventional ones. We have also revealed that A (1–40) and A (1–42) strictly recognize the individual structure.

Keywords: AGGREGATION; ALZHEIMER'S DISEASE; AMYLOID-BETA-PROTEIN; BEADS; IMMOBILIZATION; INHIBITOR; SCREENING METHOD

Document Type: Research Article

DOI: http://dx.doi.org/10.1166/jbn.2005.047

Publication date: September 1, 2005

More about this publication?
  • Journal of Biomedical Nanotechnology (JBN) is a peer-reviewed multidisciplinary journal providing broad coverage in all research areas focused on the applications of nanotechnology in medicine, drug delivery systems, infectious disease, biomedical sciences, biotechnology, and all other related fields of life sciences.
  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • Terms & Conditions
  • ingentaconnect is not responsible for the content or availability of external websites
asp/jbn/2005/00000001/00000003/art00017
dcterms_title,dcterms_description,pub_keyword
6
5
20
40
5

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more