Cytotoxicity on V79 and HL60 Cell Lines by Thiolated--Cyclodextrin-Au/Violacein Nanoparticles
Abstract:In vitro cytotoxicity of a supramolecular system composed by violacein complexed by -cyclodextrin-thiol-protected gold nanoparticles (violacein @ -CD–S(CH2)6–S–Au) on V79 and HL60 cell lines is described. The gold nanoparticles were prepared and modified in a single step involving reduction of tetrachloroaurate ions with sodium borohydride in the presence of thiol derivatized -cyclodextrin. Inclusion complexation of violacein into cyclodextrin cavities occurred by mixing an aqueous solution of the gold nanoparticles with an acetone solution of violacein, being evidenced by UV/visible absorption spectroscopy. According to cell viability measurements based on the MTT assay (3-(4,5-dimethylthiazole-2-yl)-2,5-biphenyl tetrazolium bromide) the supramolecular system was found to maintain the cytotoxic effects compared to free violacein on HL60 cells and in addition is less cytotoxic to normal (V79) cells.
Document Type: Research Article
Publication date: September 1, 2005
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