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Nanostructure Formation from a Poly(glutamic acid)-based Helix-Loop-Helix Copolymer and its Stable Encapsulation of Antitumor Reagent

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We describe herein the preparation of novel triblock architectures, consisting of two poly(L-glutamic acid) (PLGA) end blocks flanking a flexible poly(ethylene oxide) (PEO) loops (1s), and their conformational and aggregation properties in water. Dynamic light scattering and atomic force microscopy have been employed to characterize the self-assembling behavior of the peptide 1. The triblock peptide 1 self-assembled into nanoparticles that were ca. 100–200 nm in diameter only under the lower pH conditions (<7), although the nanostructures depended on the chain-length of PLGA and/or PEO segments. Circular dichroism measurements showed that these 1-nanoparticles were rich in α-helices and the helix content was considerably enhanced, compared with that for the PEO-free Pr-PLGA (2) with the same chain length. In addition, the pKa value for 1 was evaluated to be 6.8 that is somewhat higher than that of the corresponding 2 (6.0). These results indicate a strong interaction between α-helical PLGA segments, and such peptide–peptide interaction as well as the specific tertiary structure of 1 (helix-loop-helix) plays an important role in forming aggregation. Furthermore, the self-assembled 1-nanoparticle was found to act as a stable encapsulant for antitumor reagent (cis-dichlorodiammine platinum (II)) in water, demonstrating the potential of this peptide-architecture as novel biomaterials with well-defined nanostructures.
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Keywords: ANTITUMOR REAGENT; HELIX-LOOP-HELIX STRUCTURE; NANOSTRUCTURE; POLY(ETHYLENE OXIDE); POLY(L-GLUTAMIC ACID); SELF-ASSEMBLY

Document Type: Research Article

Publication date: 2005-09-01

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  • Journal of Biomedical Nanotechnology (JBN) is a peer-reviewed multidisciplinary journal providing broad coverage in all research areas focused on the applications of nanotechnology in medicine, drug delivery systems, infectious disease, biomedical sciences, biotechnology, and all other related fields of life sciences.
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