Nanostructure Formation from a Poly(glutamic acid)-based Helix-Loop-Helix Copolymer and its Stable Encapsulation of Antitumor Reagent
We describe herein the preparation of novel triblock architectures, consisting of two poly(L-glutamic acid) (PLGA) end blocks flanking a flexible poly(ethylene oxide) (PEO) loops (1s), and their conformational and aggregation properties in water. Dynamic light scattering and atomic force microscopy have been employed to characterize the self-assembling behavior of the peptide 1. The triblock peptide 1 self-assembled into nanoparticles that were ca. 100–200 nm in diameter only under the lower pH conditions (<7), although the nanostructures depended on the chain-length of PLGA and/or PEO segments. Circular dichroism measurements showed that these 1-nanoparticles were rich in α-helices and the helix content was considerably enhanced, compared with that for the PEO-free Pr-PLGA (2) with the same chain length. In addition, the pKa value for 1 was evaluated to be 6.8 that is somewhat higher than that of the corresponding 2 (6.0). These results indicate a strong interaction between α-helical PLGA segments, and such peptide–peptide interaction as well as the specific tertiary structure of 1 (helix-loop-helix) plays an important role in forming aggregation. Furthermore, the self-assembled 1-nanoparticle was found to act as a stable encapsulant for antitumor reagent (cis-dichlorodiammine platinum (II)) in water, demonstrating the potential of this peptide-architecture as novel biomaterials with well-defined nanostructures.
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Document Type: Research Article
Publication date: 2005-09-01
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