Nano- and Micro-Structured Substrates for Neuronal Cell Development
Authors: Haq, Furqan; Rao, Yeswanth L.; Keith, Charles; Zhao, Yiping; Zhang, Guigen
Source: Journal of Biomedical Nanotechnology, Volume 1, Number 3, September 2005 , pp. 313-319(7)
Publisher: American Scientific Publishers
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Abstract:
We investigated neurite development in a rat pheochromocytoma cell line (PC12) cultured on nanostructured, micro-structured and smooth substrates. On nano-structured substrates, cells continued to proliferate and developed shorter neurites and numerous filopodia as compared with smooth substrates. On micro-structured substrates, by contrast, cells ceased to proliferate and developed multiple and longer neurites. Additionally, cells grown on nano-structured substrates did not show any increase in acetylcholinesterase activity—a measure of cell differentiation activities, whereas cells grown on micro-structured substrates did. Thus by both morphological and biochemical criteria, nano-structured substrates inhibit and micro-structured substrates enhance PC12 differentiation. These results suggest the possibility of using substrates with topographical features at the nanometer or micrometer scales to control cell growth, such as using nano-substrates to promote cell proliferation and using micro-substrates to promote cell differentiation.Keywords: NANO-SUBSTRATES; MICRO-SUBSTRATES; NEURITE DEVELOPMENT; FILOPODIA; PROLIFERATION; DIFFERENTIATION
Document Type: Research article
DOI: 10.1166/jbn.2005.042
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