Skip to main content

Nano- and Micro-Structured Substrates for Neuronal Cell Development

Buy Article:

$113.00 plus tax (Refund Policy)


We investigated neurite development in a rat pheochromocytoma cell line (PC12) cultured on nanostructured, micro-structured and smooth substrates. On nano-structured substrates, cells continued to proliferate and developed shorter neurites and numerous filopodia as compared with smooth substrates. On micro-structured substrates, by contrast, cells ceased to proliferate and developed multiple and longer neurites. Additionally, cells grown on nano-structured substrates did not show any increase in acetylcholinesterase activity—a measure of cell differentiation activities, whereas cells grown on micro-structured substrates did. Thus by both morphological and biochemical criteria, nano-structured substrates inhibit and micro-structured substrates enhance PC12 differentiation. These results suggest the possibility of using substrates with topographical features at the nanometer or micrometer scales to control cell growth, such as using nano-substrates to promote cell proliferation and using micro-substrates to promote cell differentiation.


Document Type: Research Article


Publication date: September 1, 2005

More about this publication?
  • Journal of Biomedical Nanotechnology (JBN) is a peer-reviewed multidisciplinary journal providing broad coverage in all research areas focused on the applications of nanotechnology in medicine, drug delivery systems, infectious disease, biomedical sciences, biotechnology, and all other related fields of life sciences.
  • Editorial Board
  • Information for Authors
  • Subscribe to this Title
  • Terms & Conditions
  • Ingenta Connect is not responsible for the content or availability of external websites

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Partial Open Access Content
Partial Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more