Authors: Haq, Furqan; Rao, Yeswanth L.; Keith, Charles; Zhao, Yiping; Zhang, Guigen

Source: Journal of Biomedical Nanotechnology, Volume 1, Number 3, September 2005 , pp. 313-319(7)

Publisher: American Scientific Publishers

Abstract:

We investigated neurite development in a rat pheochromocytoma cell line (PC12) cultured on nanostructured, micro-structured and smooth substrates. On nano-structured substrates, cells continued to proliferate and developed shorter neurites and numerous filopodia as compared with smooth substrates. On micro-structured substrates, by contrast, cells ceased to proliferate and developed multiple and longer neurites. Additionally, cells grown on nano-structured substrates did not show any increase in acetylcholinesterase activity—a measure of cell differentiation activities, whereas cells grown on micro-structured substrates did. Thus by both morphological and biochemical criteria, nano-structured substrates inhibit and micro-structured substrates enhance PC12 differentiation. These results suggest the possibility of using substrates with topographical features at the nanometer or micrometer scales to control cell growth, such as using nano-substrates to promote cell proliferation and using micro-substrates to promote cell differentiation.

Keywords: NANO-SUBSTRATES; MICRO-SUBSTRATES; NEURITE DEVELOPMENT; FILOPODIA; PROLIFERATION; DIFFERENTIATION

Document Type: Research article

DOI: http://dx.doi.org/10.1166/jbn.2005.042

Publication date: 2005-09-01

More about this publication?

• Journal of Biomedical Nanotechnology (JBN) is a peer-reviewed multidisciplinary journal providing broad coverage in all research areas focused on the applications of nanotechnology in medicine, drug delivery systems, infectious disease, biomedical sciences, biotechnology, and all other related fields of life sciences.
• Editorial Board
• Information for Authors
• Subscribe to this Title
• Terms & Conditions
• ingentaconnect is not responsible for the content or availability of external websites

Related content

• In this: publication
• By this: publisher
• In this Subject: Technology ,  Biochemistry
• By this author: Haq, Furqan ;  Rao, Yeswanth L. ;  Keith, Charles ;  Zhao, Yiping ;  Zhang, Guigen

Website © Publishing Technology. Article copyright remains with the publisher, society or author(s) as specified within the article.

• Terms and conditions
• Privacy policy
• Information for advertisers