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Quantitative Analysis of Bacterial Aggregation Mediated by Bioactive Nanoparticles

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Polymeric nanoparticles with surface-tethered mannoses were synthesized for the binding to bacteria bearing mannose-specific adhesins, as reported previously. Presented here are experimental results that quantify the magnitude of cell aggregation mediated by the bioactive nanoparticles. The colony forming unit (CFU) assay suggested a reduction of up to 99%, consistent with the fluorescence microscopy images showing large bacterial cell clumps of hundreds to thousands of bacteria. The specificity of the bacterial-binding and potential applications of the bioactive nanoparticles as antibacterial agents are discussed.
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Document Type: Research Article

Publication date: 2005-09-01

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  • Journal of Biomedical Nanotechnology (JBN) is a peer-reviewed multidisciplinary journal providing broad coverage in all research areas focused on the applications of nanotechnology in medicine, drug delivery systems, infectious disease, biomedical sciences, biotechnology, and all other related fields of life sciences.
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