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Correlative Microscopy of Cerebellar Neuroglial Cells

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Abstract:

Perineuronal, perivascular and interfascicular astrocytes are distinguished in the three-layered structure of cerebellar cortex. Velate protoplamic astrocytes of granular layer appear as high mass dense cells with laminar processes enwraping granule and Golgi cells, and glomerular regions. With scanning electron microscopy (SEM) and SEM freeze-fracture method the neuroglial cells exhibit a glassy cytoplasm fracture face, the relief of the outer surface of endoplasmic reticulum, and cell organelles. Their nuclei display the three-dimensional aspect of nuclear chromatin strands. At transmission electron microscopy (TEM) level, mouse cerebellar Bergmann glial cells show electron lucent cytoplasm containing cell organelles, cytoskeleton and scarce profiles of rough endoplasmic reticulum. The SEM examination of human cerebellar cortex using the ethanol-cryofracturing technique display the Bergmann glial cells as satellite cells surrounding Purkinje cells. The TEM freeze-etching replicas of Bergmann glial cells show the encapsulating process around nerve cell processes of the molecular layer, the presence of intramembrane particles, and the orthogonal arrangement of intramembrane particles. With field emission SEM, the Bergmann glial cells show the cytoplasmic processes as thin, and lamellate, high mass density cytoplasm ensheathing the Purkinje cell spiny dendritic ramifications. Intense immunopositive GFAP immunoreactivity is shown in Bergmann glial cell soma and processes surrounding Purkinje cell soma, and dendritic arborization. Oligodendrocytes appear as round or oval cells closely associated with afferent mossy and climbing fibers. Confocal laser scanning microscopy observations show strong positive N-cadherin, GluR1 and CaMKII immunoreactivities. Rat cerebellar cortex stained with a fluorescently conjugated isolectin-B4 (IB4) and Alexa fluor 488, and OX-42 primary antibody reveal numerous ramified IB4-positive yuxtavascular and parenchymal microglia cells.

Keywords: ASTROCYTES; CEREBELLAR CORTEX; CORRELATIVE MICROSCOPY; IMMUNOHISTOCHEMISTRY; MICROGLIAL CELLS; OLIGODENDROCYTES

Document Type: Review Article

DOI: http://dx.doi.org/10.1166/jamr.2011.1070

Publication date: August 1, 2011

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  • Journal of Advanced Microscopy Research (JAMR) provides a forum for rapid dissemination of important developments in high-resolution microscopy techniques to image, characterize and analyze man-made and natural samples; to study physicochemical phenomena such as abrasion, adhesion, corrosion and friction; to perform micro and nanofabrication, lithography, patterning, micro and nanomanipulation; theory and modeling, as well as their applications in all areas of science, engineering, and medicine.
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