AFM Study of Cellular Structure Organelles of Lacandonia schismatica and Visualization of Images Using the Error Signal
Abstract:In this work we show the enhancement of structural details of cellular organelles of Lacandonia schismatica by means of an atomic force microscope in the feedback loop error signal mode, compared to the constant force mode which is widely used. The samples were prepared as for transmission electron microscopy. It was possible to observe some granular structures inside the interchromatin space, therefore using the feedback loop error signal mode, some features are observed with enhancement of the borders and limits of the membranes that detail with more clarity the granular structure inside the nuclei and some secretion materials taken place by enzymatic processes in the vacuoles that can not be observed in topography contact mode. The structural details that can be imaged are placed in the compact chromatin, nucleus, nucleoplasm, nucleolus, ribonucleoproteins, embryonic sack, cellular walls, and in some cells in division process. This is a very important step in order to understand the structure and role of the main nuclear sub compartments in the biology of this species.
Document Type: Research Article
Publication date: 2011-02-01
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- Journal of Advanced Microscopy Research (JAMR) provides a forum for rapid dissemination of important developments in high-resolution microscopy techniques to image, characterize and analyze man-made and natural samples; to study physicochemical phenomena such as abrasion, adhesion, corrosion and friction; to perform micro and nanofabrication, lithography, patterning, micro and nanomanipulation; theory and modeling, as well as their applications in all areas of science, engineering, and medicine.
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