Introduction: Dengue virus (DENV) causes an acute infection that may lead to death. Due to the morbidity and mortality produced, dengue infection is still a serious health problem especially in tropical countries. To this time, there is still no specific antiviral to overcome
DENV infection. Therefore, a research to find a specific antiviral for DENV is necessary. This research is aimed to evaluate the effect of Cynometra ramiflora Linn. leaf extract In Vitro on Huh7it-1 cell as a specific antiviral for DENV infection. Methods: An experimental research
was conducted in August 2014–June 2015. The leaf extract of C. ramiflora Linn. was diluted to concentration 40 μg/ml, 20 μg/ml, 10 μg/ml, 5 μg/ml, 2,5 μg/ml, and 1,25 μg/ml. Next, DENV was exposed to each concentration. The
inhibition of DENV replication (IC50) was observed using Focus Assay, while the toxicity of the extract to Huh7it-1 (CC50) was evaluated using MTT Assay. Data were analyzed using T -test and One Way ANOVA. Results: From the experiment, the value of CC50 and IC50 are 125 μg/ml
and 20,1 μg/ml, respectively. Conclusion: From the research, it is concluded that C. ramiflora Linn. extract is not toxic and has a potency as antiviral for DENV.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
Cynometra Ramiflora Linn;
Document Type: Research Article
Faculty of Medicine, Universitas Indonesia, Indonesia, Central Jakarta, Indonesia
Department of Microbiology, Faculty of Medicine, Universitas Indonesia, Indonesia, Central Jakarta, Indonesia
Publication date: 01 July 2017
More about this publication?
ADVANCED SCIENCE LETTERS is an international peer-reviewed journal with a very wide-ranging coverage, consolidates research activities in all areas of (1) Physical Sciences, (2) Biological Sciences, (3) Mathematical Sciences, (4) Engineering, (5) Computer and Information Sciences, and (6) Geosciences to publish original short communications, full research papers and timely brief (mini) reviews with authors photo and biography encompassing the basic and applied research and current developments in educational aspects of these scientific areas.
- Editorial Board
- Information for Authors
- Subscribe to this Title
- Ingenta Connect is not responsible for the content or availability of external websites