Nanospheres of Recombinant Human Bone Morphogenetic-2 and Polylactic Acid Promote the Healing of Bone Fractures In Vivo
Abstract:The healing of bone fractures is a complicated and lengthy process. Currently, many studies are devoted to studying methods of accelerating fracture healing. There are intense research efforts to develop controlled release scaffolds with ideal properties for therapeutic delivery. The progress in biomaterial development, especially nanomaterials, provides a new approach to solve this problem. In our previous study, nanospheres of recombinant human bone morphogenetic-2 (rhBMP-2) and polylactic acid (rhBMP-2-PLA-Ns) were prepared and demonstrated to be an ideal controlled release system that was able to accelerate fracture healing in vitro Due to differences between the in vivo and in vitro environments, it is essential to perform further investigations using in vivo models to assess the feasibility of this strategy. In this study, a model of rabbit mandible fracture was developed. The blank control, rhBMP-2 alone and rhBMP-2-PLA-Ns groups were divided randomly. In the blank control group, no exogenous factors were added. In the rhBMP-2 alone group, rhBMP-2 was injected at the fracture site. In the rhBMP-2-PLA-Ns group, rhBMP-2-PLA-Ns gel was coated on the fracture site. Fracture site biopsies were acquired at one, two and four weeks following surgery. Fracture biopsies were analyzed using hematoxylin-eosin staining, tetracycline fluorescence staining, VEGF mRNA in situ hybridization, and immunohistochemistry. Fractures coated with the rhBMP-2-PLA-Ns gel had increased expression of VEGF and PCNA that significantly promoted the regeneration of callus and blood vessels compared to two control groups. Therefore, we can conclude that rhBMP-2-PLA-Ns gel is capable of promoting the healing of bone fractures and that this approach may have beneficial clinical applications.
Document Type: Research Article
Publication date: 2012-03-01
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