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Measurement of Vorticella Contraction Force Using a Micropipette Technique

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We have measured the force developed by Vorticella sp., a protozoan that posses a contraction mechanism which is ATP-independent. The force measurement was performed through a setup known as Micropipette Pressure Transducing System (MPTS), a technique which is widely used to study the mechanical properties of vesicle membranes. Interestingly enough, the technique allows us to evaluate directly, to our knowledge, for the first time, the contraction force of this kind of living organism in vivo, anchored to a substrate of its living medium and not evoked by any artificial stimulus. The contraction force of Vorticella measured by this method was 117±11.74 nN, this order of magnitude is higher than the values that has been reported for a living Vorticella.
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Document Type: Research Article

Publication date: 2010-12-01

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