An Amperometric Biosensor Based on Glucose Oxidase Immobilized in a Sol–Gel Polyvinyl Alcohol/Silica Hybrid Composite Film on a Prussian Blue Modified Electrode
An amperometric glucose biosensor was fabricated by entrapment of glucose oxidase in a composite matrix of silica sol–gel and polyvinyl alcohol physically layered on top of a Prussian blue-modified Pt electrode. This biosensor functions through the detection of hydrogen peroxide produced by the glucose oxidase-catalyzed oxidation of glucose at –0.05 V. The biosensor exhibited high selectivity, and a sensitivity of 3.41 μA/mM, due to a combination of electroreduction of H2O2 at the surface of the modified Pt electrode at low potential and a sympathetic microenvironment for enzyme immobilization provided by the silica sol–gel. The biosensor had a linear response to glucose over the range 5.0×10–5 to 2.5×10–3 M, with a response time of less than 8 sec. The detection limit of the biosensor is 4.0 μM, based on a signal-to-noise ratio of 3. The biosensor exhibits satisfactory long-term stability; 85% of its original activity was retained after 30 days of storage in phosphate buffer (pH 6.9; 0.1 M) at 4 °C.
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Document Type: Research Article
Publication date: 2010-12-01
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