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An Amperometric Biosensor Based on Glucose Oxidase Immobilized in a Sol–Gel Polyvinyl Alcohol/Silica Hybrid Composite Film on a Prussian Blue Modified Electrode

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An amperometric glucose biosensor was fabricated by entrapment of glucose oxidase in a composite matrix of silica sol–gel and polyvinyl alcohol physically layered on top of a Prussian blue-modified Pt electrode. This biosensor functions through the detection of hydrogen peroxide produced by the glucose oxidase-catalyzed oxidation of glucose at –0.05 V. The biosensor exhibited high selectivity, and a sensitivity of 3.41 μA/mM, due to a combination of electroreduction of H2O2 at the surface of the modified Pt electrode at low potential and a sympathetic microenvironment for enzyme immobilization provided by the silica sol–gel. The biosensor had a linear response to glucose over the range 5.0×10–5 to 2.5×10–3 M, with a response time of less than 8 sec. The detection limit of the biosensor is 4.0 μM, based on a signal-to-noise ratio of 3. The biosensor exhibits satisfactory long-term stability; 85% of its original activity was retained after 30 days of storage in phosphate buffer (pH 6.9; 0.1 M) at 4 °C.

Document Type: Research Article


Publication date: December 1, 2010

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  • ADVANCED SCIENCE LETTERS is an international peer-reviewed journal with a very wide-ranging coverage, consolidates research activities in all areas of (1) Physical Sciences, (2) Biological Sciences, (3) Mathematical Sciences, (4) Engineering, (5) Computer and Information Sciences, and (6) Geosciences to publish original short communications, full research papers and timely brief (mini) reviews with authors photo and biography encompassing the basic and applied research and current developments in educational aspects of these scientific areas.
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