In Vitro Synthesis of Silver Nanoparticles by Marine Yeasts from Coastal Mangrove Sediment
Twelve species of marine yeasts derived from the mangrove sediment were tested for the synthesis of silver nanoparticles. Among the yeasts, Pichia capsulata exhibited the most efficient production of silver nanoparticles in culture filtrate, at a faster rate within minutes. Synthesis of silver nanoparticles in the culture filtrate was confirmed by absorption peak at 430 nm, as well under transmission electron microscope. The synthesis of silver nanoparticles was the maximum when the culture filtrate was treated with 1.5 mM AgNO3, 0.3% NaCl, maintained with pH of 6.0 and incubated at 5 °C for 24 h. The culture filtrate, precipitated with ammonium sulphate, exhibited a single protein band with a molecular weight of 55 kDa, using polyacrylamide gel electrophoresis. The work supports the possibility of using marine yeasts to achieve a faster rate of silver nanoparticle synthesis.
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Document Type: Research Article
Publication date: 2010-12-01
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