Pre- and postnatal exposure to ambient levels of urban particulate matter (PM2.5) affects mice spermatogenesis
Source: Inhalation Toxicology, Volume 23, Number 4, March 2011 , pp. 237-245(9)
Publisher: Informa Healthcare
Abstract:This work characterizes the effects of ambient levels of urban particulate matter (PM2.5) from the city of Sao Paulo on spermatogenesis using mice exposed during the embryo––fetal and/or postnatal phases of development. Parental generations (BALB/c mice) were exposed to air pollution in chambers with or without filtering PM2.5 for 4 months. Animals were mated, and half of the 1-day-old offspring were moved between chambers, which yielded prenatal and postnatal groups. Remaining offspring comprised the non-exposed and pre++postnatal exposed groups. After 90 days, the animals were sacrificed for testis collection and weighing. Optical microscopy was used for the morphometric analyses of the cell counts, spermatogenic cycle, proliferation, and apoptosis. Prenatally exposed animals presented reduced body and testicular weight with an increased gonadosomatic index (GSI). Testicular volume also decreased, as well as the tubular diameter in testes of the same animals. Proliferation, apoptosis, and spermatogenic cycle analyses showed no significant differences among groups. However, the tubules at stage VII of pre- and postnatal animals presented a reduced number of elongated spermatids. Pre++postnatal group presented higher spermatid head retention at stages VIII––XII. These results show that ambient levels of PM2.5 from Sao Paulo city affect spermatogenesis by damaging sperm production.
Document Type: Research Article
Affiliations: 1: 3Centro de Ciêências Biolóógicas, Departamento de Anatomia, Universidade Federal de Pernambuco, Pernambuco, Brazil 2: 1Laboratóório de Poluiççãão Atmosféérica Experimental, Departamento de Patologia, Faculdade de Medicina da Universidade de Sãão Paulo, Brazil
Publication date: March 1, 2011