Authors: Brvar, Miran¹; Luzar, Boštjan²; Finderle, Žarko³; Šuput, Dušan⁴; Bunc, Matjaž⁵

Source: Inhalation Toxicology, Volume 22, Number 12, October 2010 , pp. 1026-1031(6)

Publisher: Informa Healthcare

Abstract:

Introduction: The progressive clinical course with delayed neurological damage in carbon monoxide (CO) poisoning may be due to neuron apoptosis. The usefulness of hyperbaric oxygen (HBO) in different time periods after CO exposure in neuronal cell apoptosis reduction has not been evaluated thus far. The aim was to evaluate HBO efficacy in reducing neuronal apoptosis in different time periods after CO exposure.

Methods: Wistar rats were exposed to 3000 ppm CO in air for 60 min and 100% oxygen at a pressure of three bar for 30 min 0-12 h after CO exposure. The apoptosis was evaluated by immunohistochemical analysis with antibodies against activated caspase-3 and the percentage of caspase-3 positive hippocampal ganglionic cells was reported.

Results: It was shown that CO poisoning results in ganglionic cell apoptosis. The percentage of apoptotic cells in rats exposed to CO was the highest (32%), whereas the percentage of apoptotic cells in rats exposed to HBO 0 and 1 h after CO was similar with a lower percentage than rats exposed to CO. The percentage of apoptotic cells in rats exposed to HBO 3 and 5 h after CO was similar with a lower percentage than rats exposed to HBO 0 and 1 h after CO. The percentage of apoptotic cells in rats exposed to HBO 7-12 h after CO was similar with a higher percentage than rats exposed to HBO 5 h after CO.

Conclusion: HBO has a time-dependent protective effect on CO-induced neuron apoptosis with the highest efficiency at 3 and 5 h after CO poisoning.

Keywords: Carbon monoxide; hyperbaric oxygen; apoptosis; caspase-3; rats

Document Type: Research article

DOI: http://dx.doi.org/10.3109/08958378.2010.510152

Affiliations: 1: 1Poison Control Centre, University Medical Centre Ljubljana, Zaloška cesta 7, and Institute of Pathophysiology, School of Medicine, Zaloška cesta 4, Ljubljana, Slovenia 2: 2Institute of Pathology, School of Medicine, Zaloška cesta 4, University of Ljubljana, Ljubljana, Slovenia 3: 3Institute of Physiology, School of Medicine, Zaloška cesta 4, Ljubljana, Slovenia 4: 4Institute of Pathophysiology, School of Medicine, Zaloška cesta 4, Ljubljana, Slovenia 5: 5Institute of Pathophysiology, School of Medicine, Zaloška cesta 4 and Clinical Department of Cardiology, University Medical Centre Ljubljana, Zaloška cesta 7, Ljubljana, Slovenia

Publication date: 2010-10-01

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