Total and Regional Deposition of Ultrafine Particles in a Mouse Model of Allergic Inflammation of the Lung
Source: Inhalation Toxicology, Volume 20, Number 6, April 2008 , pp. 585-593(9)
Publisher: Informa Healthcare
Abstract:Epidemiological studies have shown an association between ambient particle inhalation and adverse respiratory heath effects. Inhalation of ultrafine particles (UFP, diameter <100 nm) has been suggested to contribute to exacerbation of allergic airway inflammation. Here we analyze the potential effects of allergen sensitization and challenge on total and regional deposition of UFP in the lung. Ovalbumin (OVA)-sensitized and nonsensitized mice were exposed for 1 h to ultrafine iridium particles radiolabeled with 192Ir (UF-Ir) (0.2 mg m-3) at 2 different time points either before or after allergen (OVA) challenge. Additional sensitized and nonsensitized mice were exposed to UF-Ir without allergen challenge. Lung total and regional UF-Ir deposition were calculated according to the distribution of radioactivity in the body and in the excreta during 3 days following UF-Ir inhalation. OVA-sensitized mice showed a 21% relative increase of total UF-Ir deposited fraction compared to nonsensitized mice. When UF-Ir inhalation was performed after allergen challenge, no difference in total UF-Ir deposited fraction between sensitized and nonsensitized mice was detectable. Furthermore, no differences in extrathoracic deposition or in regional particle deposition were detected between all experimental groups. This study indicates that allergen sensitization alone can affect UFP deposition in the lungs. Whether higher UFP deposition in sensitized individuals compared to nonsensitized individuals or whether other factors, like alterations in long-term clearance kinetics, contribute substantially to the susceptibility of allergic individuals to particle exposure has yet to be elucidated.
Document Type: Research Article
Affiliations: 1: Institute for Inhalation Biology, Helmholtz Zentrum München, German Research Center for Environmental Health, Munich, Germany 2: Allergy Research Group, Department of Dermatology, University Medical Center Freiburg, Freiburg, Germany 3: Institute for Inhalation Biology and Focus Network Nanoparticles and Health (NanoHealth), Helmholtz Zentrum München, German Research Center for Environmental Health, Munich, Germany
Publication date: 2008-04-01