Effect of Sulfur Dioxide Inhalation on CYP2B1/2 and CYP2E1 in Rat Liver and Lung
Authors: Qin, Guohua; Meng, Ziqiang
Source: Inhalation Toxicology, Volume 18, Number 8, July 2006 , pp. 581-588(8)
Publisher: Informa Healthcare
Abstract:Sulfur dioxide (SO 2 ) is a ubiquitous air pollutant, present in low concentrations in the urban air and in higher concentrations in the working environment. In this study, we investigated the effects of inhaled SO 2 on the O -dealkylase of pentoxyresorufin (PROD) and p -nitrophenol hydroxylases (p-NP) activities and mRNA levels of CYP2B1/2 and CYP2E1 in the lung and liver of Wistar rats. Male Wistar rats were housed in exposure chambers and treated with 14.11 ± 1.53, 28.36 ± 2.12, and 56.25 ± 4.28 mg /m 3 SO 2 for 6 h/day for 7 days, while control rats were exposed to filtered air in the same condition. The mRNAs of CYP2B1/2 and -2E1 were analyzed in livers and lungs by using reverse-transcription polymerase chain reaction (RT-PCR). Our results showed that the PROD activities and mRNA of CYP2B1/2 were decreased in livers and lungs of rats exposed to SO 2 . The p-NP activities and mRNA of CYP2E1 were decreased in lungs but not in livers of rats exposed to SO 2 . Total liver microsomal cytochrome P-450 (CYP) contents were diminished in SO 2 -exposed rats. These results lead to two conclusions: (1) SO 2 exposure can suppress CYP2B1/2 and CYP2E1 in lungs and CYP2B1/2 in livers of rats, thus modifying the liver and lung toxication/detoxication potential, and (2) the total liver microsomal CYP contents were diminished, although the activity and mRNA expression of CYP2E1 in rat livers were not affected by SO 2 exposure.
Document Type: Research article
Publication date: 2006-07-01