Acute Pulmonary Inflammation Induced by Lung Overloading with Selenium Particles: Leukocyte Response and In Situ Detection of Selenium at High Resolution
Source: Inhalation Toxicology, Volume 16, Number 14, December 2004 , pp. 901-909(9)
Publisher: Informa Healthcare
Abstract:The kinetics of the acute inflammatory response of the lung was triggered in CD-1 mice by a single intratracheal instillation of a large amount of Se (10 mg); it was studied by quantitative cytology of bronchoalveolar lavage samples, light microscopy, and scanning electron microscopy coupled with x-ray elemental microanalysis. Bronchoalveolar lavage leukocytes were mostly neutrophils and increased from 12 to 24 h of Se treatment and decreased at 72 h. Only less than half of the granulocytes showed ingested Se particles; in contrast, virtually all BAL macrophages contained Se particles. Scanning electron microscopy coupled with X-ray elemental microanalysis revealed that the intracellular Se particles were heterogeneous in size (diameters from 0.4 and up to 14μm), and that Se inclusions were sometimes accumulated at a pole of the cell. At 72 h after instillation of the particles, Se-loaded alveolar macrophages were migrated in the interstitial space of the alveoli. Se-positive regions had a focal distribution in the lung; accumulation of inflammatory cells erased the alveolar architecture of these areas of the deep lung. Our data indicates that Se overloading of the lung results in: (1) an acute inflammatory response that is dominated by neutrophils; (2) early removal of Se done mostly by alveolar macrophages, and (3) formation of focal areas of invasion of the lung parenchyma by inflammatory infiltrates.
Document Type: Research Article
Affiliations: 1: ICBAS Department of Anatomy and UMIB Abel Salazar Institute for Biomedical Sciences University of Porto, Porto, Portugal 2: Department of Medical Science Faculty of Science Burapha University, Thailand
Publication date: 2004-12-01