Respiration-Based Measurement of Lung Deposition of a Fluorescent Dextrane Aerosol in a Marmoset Monkey
Authors: Bergers, Willem; Horst, Dory; Joosen, Marloes
Source: Inhalation Toxicology, Volume 16, Number 3, March 2004 , pp. 141-146(6)
Publisher: Informa Healthcare
Abstract:A technique for measurement of respiration-based lung deposition of an aerosol was investigated and subsequently applied in a pilot study with a marmoset monkey. The technique consisted of an aerosol exposure system for a marmoset using a face mask and a previously constructed monkey chair, a method for recovery of fluorescent dextrane from lung material, and respiration measurement of the marmoset by whole-body plethysmography. In the pilot study, a ketamine-anesthetized marmoset was exposed for 20 min to an FITC–dextrane aerosol atmosphere (200 μ g/L air, particle size 1.5 μm mass median aerodynamic diameter [MMAD]). It was found that 3.4% of the inhaled aerosol was deposited in the lungs; the aerosol was distributed over the lung lobes with an higher concentration at the distal side.
Document Type: Research Article
Affiliations: TNO Prins Maurits Laboratory, Division Chemical and Biological Protection, Rijswijk, The Netherlands
Publication date: 2004-03-01