Left-sided mouse intubation: description and evaluation
Source: Experimental Lung Research, Volume 36, Number 1, February 2010 , pp. 25-30(6)
Publisher: Informa Healthcare
A method of left main bronchus intubation was developed based on a wire guide–––based microscopic endotrachael mouse intubation technique. The authors used a 22 G ××× 1 inch catheter elongated by a 38-mm silicone tube, and a wire guide with a tag to assign the length of the wire completely covered by the silicon tube. The isoflurane-anesthetized mouse was hung perpendicularly with its incisors on a thread and transorally intubated under strict vision with the wire guide tip advanced 3 mm out of the catheter. Then the catheter was advanced about 6 to 8 mm into the trachea. Afterwards the wire guide was redrawn to the level of the catheter tip (blue tag on the wire guide appeared at the upper end of catheter) to prevent injury. Then the neck was pushed into a right lateral flexion with one finger against a foam block fixed on the vertical plate, causing a straight distance between mouth and left main bronchus. This positioning allows to gently advance the catheter into the left main bronchus by another about 20 mm, using the wire guide with its tip just within the tube, to achieve there a wedge position with gentle pressure.The technique had a success rate of more than 80%%% in 81 mice weighing 23 to 48 g. It may be of interest for unilateral lung intervention, e.g., with injurious substances or with drugs.
Document Type: Research Article
Affiliations: 1: 1Internal Medicine V/Pulmonary Division, and Clinical and Experimental Surgery, University Hospital Homburg/Saar, University of Saarland, Germany 2: 2Department of Paediatric Surgery, University Children's Hospital, Züüürich, Switzerland 3: 3Section of Experimental Pneumology, Research Center Borstel, Leibnitz Center for Medicine and Biosciences, Borstel, Germany 4: 4Institute of Anatomy, University of Bern, Switzerland 5: 5Roche Pharma AG, Grenzach-Wyhlen, Germany 6: 6Pulmonary Division, University Hospital Inselspital, University of Bern, Switzerland
Publication date: 2010-02-01