Authors: Orel, V.¹; Kudryavets, Yu.²; Satz, S.³; Bezdenezhnih, N.²; Danko, M.¹; Khranovskaya, N.¹; Romanov, A.¹; Dzyatkovskaya, N.¹; Burlaka, A.²

Source: Drug Delivery, Volume 12, Number 3, May-June 2005 , pp. 171-178(8)

Publisher: Informa Healthcare

Abstract:

Targeting of mechanochemically activated doxorubicin (MA DOXO) nanoparticles, conventional doxorubicin, and electromagnetic irradiation (EMI) at A-549 lung carcinoma cells in vitro was investigated. Conventional DOXO was micronized using an input energy of 20 W/g for 5 min resulting in positively charged MA DOXO particles 10 times smaller than conventional DOXO. Mechanochemical activation gives rise to additional free quinone radicals. High performance liquid chromatograph analyses demonstrate that conventional and MA DOXO are quantitatively similar. Tumor cells were exposed to 40 MHz electromagnetic irradiation at a power density of 2 W/cm 2 . The lethal dose LD 50 values of MA DOXO were 5 times greater than conventional doxorubicin. MA DOXO in combination with EMI at 37°C demonstrates improved drug delivery to A-549 human lung carcinoma and greater cell kill than does conventional DOXO.

Keywords: A-549 Human Lung Carcinoma Cells; Doxorubicin; Electromagnetic Irradiation; Mechanical Activation; Nanoparticles

Document Type: Research article

DOI: http://dx.doi.org/10.1080/10717540590932007

Affiliations: 1: Institute of Oncology, Academy of Medical Sciences, Kyiv, Ukraine 2: R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, National Academy of Sciences of Kyiv, Ukraine 3: Bio-Nucleonics, Inc., Miami, Florida, USA

Publication date: 2005-05-01

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