Authors: M. Wojewódzka; M. Kruszewski; B. Sochanowicz; I. Szumiel

Source: International Journal of Radiation Biology, Volume 80, Number 7, July 2004 , pp. 473-482(10)

Publisher: Informa Healthcare

Abstract:

Purpose: To investigate the role of poly(ADP-ribosylation) in DNA double-strand break repair and fixation in murine lymphoma L5178Y (LY) sublines, LY-R and LY-S, and a pair of Chinese hamster ovary lines: wild-type and mutant xrs6 cells, that have differences in repair competence and degree of radiosensitization with poly(ADP-ribosylation) inhibitors.

Materials and methods: Cells (asynchronous, logarithmic phase) were pre-incubated with 2 mM aminobenzamide at 37 or 25°C, X-irradiated with 10 Gy and allowed to repair DNA breaks for 15, 60 and 120 min at 37 or 25°C. The remaining double-strand break were estimated by the neutral comet assay.

Results: At 37°C, no effect of AB treatment on the repair kinetics was observed either in xrs6 or Chinese hamster ovary (wild-type) cells. In contrast, aminobenzamide decreased the repair of double-strand break in the LY-S line but not the LY-R line, in agreement with the previously observed radiosensitization of LY cells by poly(ADP-ribosylation) inhibition. However, double-strand break rejoining in the repair competent cell lines, Chinese hamster ovary and LY-R, also was affected by aminobenzamide when the post-irradiation incubation was carried out at 25°C. Analysis of these results together with earlier data on LY-S cells have been interpreted in terms of Radford's model of radiation damage fixation.

Conclusion: The reported results indicate that poly(ADP-ribosylation) can be an important modulator of the conversion of DNA damage to lethal events.

Document Type: Research article

DOI: http://dx.doi.org/10.1080/09553000410001724216

Affiliations: 1: Department of Radiobiology and Health Protection Institute of Nuclear Chemistry and Technology Dorodna 16 PL-03-195 Warsaw Poland

Publication date: 2004-07-01

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