Authors: Siwale, Rodney¹; Oettinger, Carl²; Balakrishna Pai, S.¹; Addo, Richard¹; Uddin, Nasir¹; Siddig, Aladin³; D'Souza, Martin¹

Source: Journal of Microencapsulation, Volume 26, Number 5, August 2009 , pp. 411-419(9)

Publisher: Informa Healthcare

Abstract:

Catalase in albumin microspheres were formulated for intravenous administration to antagonize the effects of over-production of reactive oxygenated species (ROS) such as hydrogen peroxide (H2O2) in septic shock. The aim was to increase effective half-life of catalase and take advantage of the phagocytic uptake of the encapsulated catalase by the vascular endothelium. Catalase microspheres were prepared by spray-drying. The microspheres were evaluated for particle size, particle shape and surface morphology by scanning electron microscopy (SEM), drug encapsulation efficiency, chemical stability, thermal stability and in vitro drug release characteristics. The microspheres had a mean particle size of 4.7 ± 2 µm, optimal for phagocytic uptake, as demonstrated by Makino et al. SEM revealed that microspheres were spherical with smooth surface morphology. An encapsulation efficiency of 91.5 ± 3% was achieved and the encapsulated catalase was chemically and thermally stable. Application of in vitro drug release data to the Higuchi kinetic equation indicated matrix diffusion-controlled catalase release from albumin microspheres.

Keywords: Microspheres; albumin; catalase

Document Type: Research article

DOI: http://dx.doi.org/10.1080/02652040802420409

Affiliations: 1: Mercer University College of Pharmacy and Health Sciences, Atlanta, Georgia, USA 2: Dialysis Clinic Inc., Atlanta, Georgia, USA 3: University of Charleston School of Pharmacy, Charleston, WV, USA

Publication date: 2009-08-01

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