Authors: Nayak, Bismita¹; Panda, Amulya²; Ray, Pratima³; Ray, Alok¹

Source: Journal of Microencapsulation, Volume 26, Number 2, March 2009 , pp. 154-165(12)

Publisher: Informa Healthcare

Abstract:

Polylactide (PLA) and polylactide-co-glycolide (PLGA) particles entrapping rotavirus (strain SA11) were formulated using a solvent evaporation technique. To minimize denaturation of viral antigen during the emulsification process, serum albumin was used as a stabilizer. Use of NaHCO3 and sucrose during the primary emulsification step resulted in uniform stabilized particles entrapping rotavirus. Sonication during the primary emulsion and homogenization during the secondary emulsion process resulted in particles of sizes 2-8 μm, whereas nanoparticles were formed when sonication was used during both primary and secondary emulsion processes. Scanning electron and atomic force microscopy showed uniform pores and roughness throughout the polymer particle surface. Single dose oral immunization with 20 μg of antigen entrapped in PLA particles elicited improved and long-lasting IgA and IgG antibody titer in comparison to the soluble antigen. The study shows results illustrating the usefulness of polymeric microparticles as a potential oral delivery system for rotavirus vaccine.

Keywords: Microparticle; biodegradable polymers; rotavirus; immune response; IgA and IgG antibody titer

Document Type: Research article

DOI: http://dx.doi.org/10.1080/02652040802211709

Affiliations: 1: Centre for Biomedical Engineering, Indian Institute of Technology Delhi, New Delhi, India,Centre for Biomedical Engineering, All India Institute of Medical Sciences, New Delhi, India 2: Product Development Cell, National Institute of Immunology, New Delhi, India 3: Department of Pediatrics, Centre for Diarrheal Disease Research, All India Institute of Medical Sciences, New Delhi, India

Publication date: 2009-03-01

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