Biological and clinical significance of p75NTR expression in laryngeal squamous epithelia and laryngocarcinoma
Source: Acta Oto-Laryngologica, Volume 132, Number 3, March 2012 , pp. 314-324(11)
Publisher: Informa Healthcare
Abstract:<title>Abstract</title> Conclusion: The apparent features of p75 neurotrophin receptor (p75NTR) expression indicated that p75NTR would serve as a potential stem cell marker for normal human laryngeal squamous epithelia. In human laryngeal squamous cell carcinoma (LSCC) p75NTR is differentially expressed. The abnormal expression and distribution of p75NTR may indicate malignant transformation. Objective: To investigate the expression of p75NTR and its possible roles in normal laryngeal squamous epithelia and LSCC. Methods: We used immunohistochemistry methods to examine normal laryngeal epithelia, para-cancer mucosa with dysplasia, laryngeal papilloma, and LSCC specimens for the expression of p75NTR, nerve growth factor (NGF), -tyrosine kinase receptor (TrkA), p63, and Ki67. Immunocytochemistry and flow cytometry were used to examine the expression of p75NTR in Hep-2 cells. Results: The expression of p75NTR was only located in basal cells of normal laryngeal epithelia, consistent with the staining features of epithelial stem cells as evidenced by parallel staining of p63, a putative keratinocyte stem cell marker. p75NTR is differentially expressed in LSCC, although no significant relationship was found with many clinicopathologic factors, this expression and distribution may correlate to malignant transformation and tumor proliferation. Co-expression of p75NTR and CD133 was confirmed, showing the association of p75NTR-positive cells with cancer stem cells in Hep-2 cells.
Document Type: Research article
Affiliations: 1: 1 Department of Otorhinolaryngology Head and Neck Surgery, Center for Otorhinolaryngological Diseases 2: 2 Department of Pathology, Bethune International Peace Hospital, Shijiazhuang, China
Publication date: 2012-03-01