Authors: Li, Xiaoming¹; Shen, Yupeng¹; Di, Bin¹; Li, Jun¹; Geng, Jiangqiao¹; Lu, Xiuying¹; He, Zhanguo²

Source: Acta Oto-Laryngologica, Volume 132, Number 3, March 2012 , pp. 314-324(11)

Publisher: Informa Healthcare

Abstract:

<title>Abstract</title>

Conclusion: The apparent features of p75 neurotrophin receptor (p75^NTR) expression indicated that p75^NTR would serve as a potential stem cell marker for normal human laryngeal squamous epithelia. In human laryngeal squamous cell carcinoma (LSCC) p75^NTR is differentially expressed. The abnormal expression and distribution of p75^NTR may indicate malignant transformation. Objective: To investigate the expression of p75^NTR and its possible roles in normal laryngeal squamous epithelia and LSCC. Methods: We used immunohistochemistry methods to examine normal laryngeal epithelia, para-cancer mucosa with dysplasia, laryngeal papilloma, and LSCC specimens for the expression of p75^NTR, nerve growth factor (NGF), -tyrosine kinase receptor (TrkA), p63, and Ki67. Immunocytochemistry and flow cytometry were used to examine the expression of p75^NTR in Hep-2 cells. Results: The expression of p75^NTR was only located in basal cells of normal laryngeal epithelia, consistent with the staining features of epithelial stem cells as evidenced by parallel staining of p63, a putative keratinocyte stem cell marker. p75^NTR is differentially expressed in LSCC, although no significant relationship was found with many clinicopathologic factors, this expression and distribution may correlate to malignant transformation and tumor proliferation. Co-expression of p75^NTR and CD133 was confirmed, showing the association of p75^NTR-positive cells with cancer stem cells in Hep-2 cells.

Keywords: Stem cell marker; cancer stem cells; CSCs; immunohistochemistry

Document Type: Research article

DOI: http://dx.doi.org/10.3109/00016489.2011.639086

Affiliations: 1: 1 Department of Otorhinolaryngology Head and Neck Surgery, Center for Otorhinolaryngological Diseases 2: 2 Department of Pathology, Bethune International Peace Hospital, Shijiazhuang, China

Publication date: 2012-03-01

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